NIH Roadmap Membrane Protein Production and Technologies Meeting
November 1-2, 2007
at The Scripps Research Institute, La Jolla, CA
Mission:
The first expanded NIH Roadmap meeting to include all funded Roadmap Membrane Protein investigators will be held at TSRI November 1-2, 2007. The meeting will provide a unique opportunity for members of the two Roadmap membrane protein centers (JCIMPT and MPEC) as well as those from projects funded through the RFA Membrane Protein Production and Structure Determination (RFA-RM-04-026 and RFA-RM-07-003) to discuss the current status of their projects. Presentation and discussion will deal with progress as well as barriers to progress that could transform membrane protein structure determination processes to produce results comparable to that now seen in studies of soluble proteins.
Logistical Information:
Please Register before July 1, 2007.
To register, click here: Registration Form
Logistical information regarding transporation, housing and other arrangements will be updated soon. Please check back or contact Angela L. Walker at alwalker@scripps.edu.
Meeting Agenda:
To download a PDF version of the agenda, click here: RMI_Meeting_Agenda.pdf.
Thursday, November 1st, 2007: Day 1
Welcome and Introduction
8:00a-8:30a Raymond Stevens, Ph.D.
Professor, Departments of Molecular Biology and Chemistry, TSRI and PI, Joint Center for Innovative Membrane Protein Technologies (JCIMPT)
-and-
Robert Stroud, Ph.D.
Professor, Departments of Biochemistry and Biophysics, and Pharmaceutical Chemistry, UCSF and PI, Membrane Protein Expression Center (MPEC)
Expression Studies Session
Session Chair: Kurt Wüthrich, Ph.D.
Ida M. Green Professor of Structural Biology, TSRI and 2002 Nobel Laureate in Chemistry
8:30a-9:00a James Bowie, Ph.D.
Professor, Department of Chemistry and Biochemistry, UCLA
“Improving Membrane Protein Expression in E. coli”
9:00a-9:20a Philippe Gros, Ph.D.
Professor, Department of Biochemistry, McGill University
“Expression of ABC Transporters in Pichia Pastoris”
9:20a-9:40a John Hunt, Ph.D.
Associate Professor, Department of Biological Sciences, Columbia University
“A Chemical Genetics Approach to Improved IMP Expression in E. Coli”
9:40a-10:10a Senyon Choe, Ph.D.
Professor, Department of Structural Biology, The Salk Institute
“Mistic and Misticated Proteins”
10:10a-10:40a BREAK & POSTER SESSION
10:40a-11:10a Stephen Kent, Ph.D.
Professor, Department of Biochemistry and Molecular Biology, University of Chicago
“Total Chemical Synthesis of Membrane Proteins”
11:10a-11:40a Geoffrey Chang, Ph.D.
Associate Professor, Department of Molecular Biology, TSRI
“Cell Free Expression of Membrane Proteins”
11:40a-12:10p David Julius, Ph.D.
Professor and Vice Chair of Cellular & Molecular Pharmacology, UCSF
“Expression in HEK Cells”
12:10p-1:30p Lunch BREAK & POSTER SESSION
Stability Studies Session
Session Chair: M.G. Finn, Ph.D.
Professor, Department of Chemistry, TSRI
1:30p-2:00p Philip Laible, Ph.D.
Biophysicist, Bioscience Division, Argonne National Laboratory
“Biomimetic Reagents that Promote Membrane Protein Stability and Crystallization”
2:00p-2:30p Qinghai Zhang, Ph.D.
Assistant Professor, Department of Molecular Biology, TSRI
“Developing Reagents for Stabilization of Membrane Proteins”
2:30p-3:00p Ronald Kaback, M.D.
Depts. of Physiology and Microbiology, Immunology & Molecular Genetics, UCLA
“Importance of Lipids and Detergents”
3:00p-3:30p BREAK & POSTER SESSION
Crystallization Studies Session
Session Chair: Peter Kuhn, Ph.D.
Associate Professor, Department of Cell Biology, TSRI
3:30p-4:00p Martin Caffrey, Ph.D.
Adjunct Professor, Department of Chemistry, The Ohio State University, and Professor of Membrane Structural Biology, University of Limerick, Ireland
“Membrane Protein Crystallization in Lipidic Mesophases. Recent Successes.”
4:00p-4:30p Rustem Ismagilov, Ph.D.
Associate Professor, Department of Chemistry and Institute for Biophysical Dynamics, University of Chicago
“Crystallization of Membrane Proteins in Microfluidic Nanoliter Droplets (plugs)”
4:30p-4:50p Paul Kenis, Ph.D.
Assistant Professor, Department of Chemical Biology and Biomolecular Engineering, University of Illinois at Urbana-Champaign
“Microfluidic Platforms for Membrane Protein Crystallization”
4:50p-5:10p Patrick Loll, Ph.D.
Associate Professor, Department of Biochemistry and Molecular Biology, Drexel University
“Mapping the Crystallization Slot for Membrane Proteins”
5:10p-5:30p Michael Wiener, Ph.D.
Associate Professor, Department of Molecular Physiology and Biological Physics, University of Virginia
“Improved Methods for Membrane Protein Crystallization”
5:45p-8:30p BREAK Reception & Dinner
Friday, November 2nd, 2007: Day 2
International Efforts Session
Session Chair: Qinghai Zhang, Ph.D.
Assistant Professor, Department of Molecular Biology, TSRI
8:00a-8:30a Hartmut Michel, Ph.D.
Director, Department of Molecular Membrane Biology, Max-Planck Institut für Biophysik
“Structural Proteomics of Membrane Proteins”
8:30a-9:00a So Iwata, Ph.D.
David Blow Chair of Biophysics and Director of Centre for Structural Biology, Imperial College of London
“Yeast Expression System and Antibody Crystallization of Human Membrane Proteins”
9:00a-9:30a Doug Rees, Ph.D.
Professor, Department of Chemistry, California Institute of Technology and Howard Hughes Medical Institute Investigator
“Structural Biology of Membrane Proteins”
9:30a-10:00a BREAK & POSTER SESSION
Biophysical Characterization
Session Chair: Mark Yeager, M.D., Ph.D.
Professor, Departments of Cell Biology and Molecular Biology, TSRI
10:00a-10:30a Ronald Milligan, Ph.D.
Professor, Department of Cell Biology and Director, Center for Integrative Molecular Biosciences, TSRI
“Electron Microscopy of Membrane Proteins”
10:30a-11:00a Stanley Opella, Ph.D.
Professor, Department of Chemistry and Biochemistry, UCSD
“Membrane Protein Structures in Phospholipid Bilayers by Solid State NMR”
11:00a-11:30a Chad Rienstra, Ph.D.
Assistant Professor, Department of Chemistry, University of Illinois at Urbana-Champaign
“3D and 4D Magic-Angle Spinning Solid-State NMR of Integral Membrane Protein Complexes”
11:30a-12:00p Kurt Wüthrich, Ph.D.
Ida M. Green Professor of Structural Biology, TSRI and 2002 Nobel Laureate in Chemistry
“NMR Analysis of Membrane Proteins”
12:00p-1:00p Lunch BREAK & POSTER SESSION
Functional Studies Session
Session Chair: Geoffrey Chang, Ph.D.
Associate Professor, Department of Molecular Biology, TSRI
1:00p-1:20p Juha-P. Himanen, Ph.D.
Senior Research Scientist, Laboratory of Structural Neuroscience, Memorial Sloan-Kettering Cancer Center
“Semi-synthetic Reconstitution of the Eph Receptor Tyrosine Kinase for Structural and Functional Studies”
1:20p-1:40p Brian Kobilka, M.D.
Professor, Departments of Medicine, and Molecular and Cellular Physiology, Stanford University School of Medicine
“Progress in Efforts to Crystallize the b2 Adrenoceptor”
1:40p-2:00p Francesca Marassi, Ph.D.
Associate Professor, Department of Apoptosis and Cell Death Reserach, The Burnham Institute
“Structure of a b-Barrel Membrane Protein in Lipid Bilayer Membranes”
2:00p-2:30p Gerhard Wagner, Ph.D.
Elkan Rogers Blout Professor of Biological Chemistry and Molecular Pharmacology, Harvard Medical School
“Studies of the Mitochondrial Outer Membrane Protein VDAC1: Preparation, NMR Structural Characterization, and Interactions”
2:30p-2:50p Dan-Neng Wang, Ph.D.
Associate Professor, Skirball Institute of Molecular Medicine and Department of Cell Biology, New York University School of Medicine
“Crystallization of Neurotransmitter Transporter Homologs”
2:50p-3:20p BREAK & POSTER SESSION
Conclusion and Farewell
3:20p-4:30p Raymond Stevens, Ph.D.
Robert Stroud, Ph.D.
And NIH Staff
“Round Table Discussion of Membrane Protein Priorities for 2007-2008”
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